Cell Manipulation as a core interdisciplinary of biological science

Cell manipulation is a technique of genetic transfer; it helps in the prevention and treatment of different diseases. Cell manipulation was first demonstrated by Bell laboratories with the use of optical tweezers, laser and infrared lights. The direct transfer of photon caused the movement of micron-sized dielectric particles to a non-absorbing particle during refraction and reflection, that is, the radiation pressure. Two forces work for trapping the cell they are the scattering force that controls the motion of particles and the other is the gradient force where the particles are trapped transversely. It is a wide aspect of study in cell and tissue biology or bioengineering.

The main objective of cell manipulation is to study the culture of mammalian cells, their growth, and the dispersal of cells that are derived from animal tissues, with an appropriate surface, proper nutrients to adapt them in a different suitable environment. Cell micropatterning and manipulation are currently representing the basic steps to perform drug testing experiments for understanding the biochemical process, microfluidic dynamics for medical applications. Cell manipulation is quite versatile in nature and can be performed on bacteria, yeast, in vivo, plants, mammalian cells, biomolecules, and many other bioparticles. After performing this technique it has been observed that in vitro assays have increased the efficiency of cells because of the cell manipulation and micropatterning.

Cell manipulation is mainly a part of microfluidics and they are used to manufacture different medicines and to diagnose the diseases.  There are certain challenges that are faced in cell manipulation like the small size of the cells and the levels of target biomolecules are also low concentrated, so it becomes difficult to analyze especially in single-cell manipulation.

Advance aspects of single-cell manipulation 

-              Based on micropatterns, cell trap and flow of cytometric, the microfluidic single-cell manipulation analysis is conducted.

-              Based on the chain reaction method, analysis of mass spectrometry, electrochemical, fluorescence, and polymerase, the detection is done.

Single-cell analysis has its application on small fields for molecular detection, multidrug resistance analysis, protein analysis and cell sequencing along with droplet microfluidics.

There are different techniques for conducting cell manipulation. Those techniques include Inkjet Cell Printing, Optical and Optoelectronic Tweezers, Laser-Based Cell Patterning, Electrokinetic Forces also known as Dielectrophoresis, Magnetic Cell Manipulation, cell patterning in microfluidic devices.

 

Achievement of cell manipulation in the medical field

Inside the microfluidic devices, the patterning and manipulating of cells have an enormous application. According to the scientists, a method to pattern cell culture in microfluid devices has helped in achieving the binding and sterilization of human umbilical vein endothelial cells (HUVEC), MDA-MB-231 breast cancer cells, and NIH 3T3 mouse fibroblasts.

These techniques are extensively followed by a manufacturer of surgical instruments WPI in the USA who made these processes a bit easier with their updated and better accuracy medical devices. They can help up to make your cell manipulation laboratory much more updated to make your gene transfer techniques to conduct easily and in a better manner.